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Worcestershire Acute Hospitals NHS Trust
Urine Culture (UF)
Test information
Urine samples are processed using Urine Image analysis. This determines whether a patient has a Urinary tract infection through detection or White Blood cells, Red Blood Cells and Bacteria. Where there is evidence of infection by this test, the sample will be cultured and sensitivity testing performed. 
For casts and crystals please request additionally on the request as they are not a routine UTI investigation, but may be useful in diagnosis of pyelonephritis (Casts) or organ dysfunction or kidney stones (Crystals). 
For these a manual microscopy would be performed.
Compliance with postal and transport regulations is essential. Specimens should be in CE 
marked leak proof specimen containers. 
Ideally, specimens should be transported and processed within 4 hours unless boric acid preservative is used.   
Use of boric acid or refrigeration will minimise deterioration up to the following limits 
Plain Universal: Testing within 4hours of collection or 48 if refrigerated 
Boric acid: Testing within 96hours (4 days) 
Special requirements
Boric acid containers: must be filled to the line according to manufacturers instructions. 
Directions for use of monovette: 
1. Remove stopper and keep for later use 
2. Attach plastic straw, insert into container and fill monovette to the bottom of the label 
4. To empty straw, hold monovette in upright position and pull plunger backwards to the bottom of the tube 
5. Remove plastic straw, break off plunger and replace cap
Tube type
100ml green topped container for urine collection, 15ml Monovette for sending urine for investigation to lab, 30ml plain universal in event of small sample. 
The concentration of Boric Acid has been shown to be inhibitory to bacteria if the volume is below 10 ml. If less than 10 ml urine use a white topped universal and send at least 1 ml.
Green topped collection cup is to be used to collect urine specimen.
Minimum volume
10ml. If the sample if small, please send at least 1ml in a white topped universal container.
TAT Inpatient (urgent)
Negatives : 1 day Positives 2 - 3 days
TAT GP/Outpatient
Negatives : 1 day Positives 2 - 4 days
Referral lab
Page last updated: 11 January 2022